Lectin binding in the choroid plexuses of the chick embryo.
نویسندگان
چکیده
In the present research we have studied the distribution and the changes of the oligosaccharidic component of the glycoconjugates during the development of the choroids plexuses of the 3rd ventricle and of the lateral ventricles in chick embryos and in 3-day-old chicken. For this purpose a battery of seven horseradish peroxidase labelled lectins was used. Lectin histochemistry has shown that starting from early stages of choroid plexuses development, all its components, epithelial cells, mesenchyme and endothelial cells of the capillary vessels, show lectin reactivity. In the present paper the possible role played by the sugar residues of the glycoconjugates in the apical portion and in the cytoplasm of the choroid epithelial cells and in the basement membrane is discussed.
منابع مشابه
Lectin binding in the ependymal cells of the cephalic portion of the nervous system in the chick embryo.
The content, distribution and changes of the glycoconjugates oligosaccharides in the ependymal cells of the cephalic portion of the nervous system, in the chick embryo from 5 days of incubation till hatching and in the 3 days old chicken, were investigated. For this purpose a battery of six HRP-conjugated lectins were used (WGA, SBA, UEA I, LTA, PNA, ConA). Enzyme and chemical treatments were p...
متن کاملChanges in the sensitivity of chick fibroblasts to Ricinus lectin (RCA I) toxicity in relation to the stage of embryo development.
The toxic effect of Ricinus lectin RCA I, as estimated by the inhibition of [3H]leucine incorporation, was investigated on chick-embryo fibroblasts at different stages of development. There appeared to be a differential susceptibility of chick-embryo fibroblasts to lectin RCA I. Fibroblasts from 16-day embryos were the most sensitive to its toxic effect in terms of both concentration and time, ...
متن کاملمطالعه هیستوشیمی و لکتین هیستوشیمی صلبیه و مشیمیه در طی روند تکامل چشم
Background and purpose: Sclera and choroid as external and middle tunic of the eye, originate from the mesenchyme surrounding the optic cup. Neural crest cells have major contribution in mesenchyme development. Main part of transmitter signals in eyes development are extracellular matrix components and cell surface glycoconjugates. The purpose of this study was to recognize extracellular m...
متن کاملAn Alkaline Phosphatase Lacking Wheat Germ Agglutinin Binding Sites Useful Enzyme for Lectin Assays with Comparable Activity to the Calf Enzyme
Despite the availability of various alkaline phosphatase (ALP) isoenzymes, the calf enzyme is being used in current enzyme assays as the detector enzyme. The glycosylation pattern of this enzyme makes it a suitable ligand for binding to wheat germ agglutinin lectin (WGA). As a result of this property, the enzyme can not be used as a conjugate with this lectin, and the calf enzyme conjugates can...
متن کاملP 144: Sunflower Mannose binding Lectin-Associated Serine Protease Inhibitor-1 (SFMI-1) and -2: Significant Inhibitors of Mannose binding Lectin Pathway which Helps in Multiple Sclerosis Treatment
One of the important parts of innate immunity is complement system that occurs in three different ways; the classic, the alternative and the lectin pathway. The four pattern recognition molecules that have been identified till now are Mannose binding lectin (MBL), a component of lectin pathway, and three ficolins (ficolin1,-2 and -3) which compound to the carbohydrates of the cell surface. MBL ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Italian journal of anatomy and embryology = Archivio italiano di anatomia ed embriologia
دوره 108 3 شماره
صفحات -
تاریخ انتشار 2003